Research Article
Exosomal miR-22-3p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE-Cadherin
Figure 2
Exosomal miR-22-3p derived from CRSwNP regulates the vascular permeability in vitro. (a) Confocal microscopy analysis of PKH67-labeled NLF-derived exosomes uptaken by HUVECs following coculture for 3h (scale bars, 10μm). Blue: Hoechst staining; green: PKH67-labeled exosomes. (b) Tubule permeability of HUVECs treated with different exosomes was measured by in vitro permeability assay. Ctrl vs. NP. Student’s test. and . (c) qRT-PCR of miR-22-3p expression in NP-exo and Ctrl-exo. (d) The representative fluorescence figures of HUVECs transfected with miR-22-3p. (e) Transfection efficiency of miR-22-3p was measured by qRT-PCR. One way ANOVA. . (f) Effects of different levels of miR-22-3p on HUVECs permeability. Mimic vs. NC. Two-way ANOVA. , , and . (g) Representative electron microscopy image of exosomes isolated from supernatant of pHNECs. (h) Nanoparticle tracking analysis of pHNECs-exo. (i) Western blot analysis of exosomal markers. (j) Forty-eight hours after treatment with exosomes isolated from supernatant of transfected pHNECs, miR-22-3p levels of HUVECs were measured by qRT-PCR. One-way ANOVA. and . (k) Effects of different levels of exosomal miR-22-3p on HUVEC permeability. Mimic vs. NC. Two-way ANOVA. , , and . Data are presented as of at least three independent experiments.
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