Effect of Saffron Extract and Crocin in Serum Metabolites of Induced Obesity Rats
Table 2
List of pathways found by qualitative MetPA analysis using MetaboAnalyst 4.0 in OBSF80.
Ranking
Pathway
Total
Expected
Hits
% found
Raw
Impact
3
Pyruvate metabolism
22
0.13
2
9.1
0.29
4
Alanine, aspartate, and glutamate metabolism
28
0.17
2
7.1
0.00
6
Glycine, serine, and threonine metabolism
34
0.20
2
5.9
0.05
1
Synthesis and degradation of ketone bodies
5
0.03
1
20.0
0.00
2
Taurine and hypotaurine metabolism
8
0.05
1
12.5
0.43
5
Butanoate metabolism
15
0.09
1
6.7
0.00
7
Starch and sucrose metabolism
18
0.11
1
5.6
0.42
9
Selenocompound metabolism
20
0.12
1
5.0
0.00
8
Citrate cycle (TCA cycle)
20
0.12
1
5.0
0.05
10
Glycolysis/gluconeogenesis
26
0.16
1
3.8
0.10
11
Galactose metabolism
27
0.16
1
3.7
0.03
12
Glyoxylate and dicarboxylate metabolism
32
0.19
1
3.1
0.00
13
Cysteine and methionine metabolism
33
0.20
1
3.0
0.00
14
Arginine and proline metabolism
38
0.23
1
2.6
0.00
15
Tyrosine metabolism
42
0.25
1
2.4
0.00
16
Primary bile acid biosynthesis
46
0.27
1
2.2
0.02
17
Aminoacyl-tRNA biosynthesis
48
0.29
1
2.1
0.00
Pathways are ranked by percent compound found in each pathway, which was determined by dividing the number of hits by the total number of compounds in the pathway. The number of the expected compounds was determined by overrepresentation analysis in MetPA, which uses hypergeometric testing to calculate the number of compounds expected to be in each pathway by chance alone. Raw values were determined based on the number of hits and total number of compounds in the pathway. The pathway impact was found using relative betweenness centrality pathway topology analysis, which calculates metabolite importance based on their position in the pathways.
