Effect of Saffron Extract and Crocin in Serum Metabolites of Induced Obesity Rats

Table 3

List of pathways found by qualitative MetPA analysis using MetaboAnalyst 4.0 in OBCR80.

Ranking	Pathway	Total	Expected	Hits	% found	Raw	Impact
4	Glycine, serine, and threonine metabolism	34	0.14	2	5.9		0.05
1	Synthesis and degradation of ketone bodies	5	0.02	1	20.0		0.00
2	Taurine and hypotaurine metabolism	8	0.03	1	12.5		0.43
3	Butanoate metabolism	15	0.06	1	6.7		0.00
5	Starch and sucrose metabolism	18	0.07	1	5.6		0.42
6	Citrate cycle (TCA cycle)	20	0.08	1	5.0		0.05
7	Pyruvate metabolism	22	0.09	1	4.5		0.21
8	Glycolysis/gluconeogenesis	26	0.10	1	3.8		0.10
9	Galactose metabolism	27	0.11	1	3.7		0.03
10	Alanine, aspartate, and glutamate metabolism	28	0.11	1	3.6		0.00
11	Glyoxylate and dicarboxylate metabolism	32	0.13	1	3.1		0.00
12	Cysteine and methionine metabolism	33	0.13	1	3.0		0.00
13	Arginine and proline metabolism	38	0.15	1	2.6		0.00
14	Tyrosine metabolism	42	0.17	1	2.4		0.00
15	Primary bile acid biosynthesis	46	0.18	1	2.2		0.02

Pathways are ranked by percent compound found in each pathway, which was determined by dividing the number of hits by the total number of compounds in the pathway. The number of the expected compounds was determined by overrepresentation analysis in MetPA, which uses hypergeometric testing to calculate the number of compounds expected to be in each pathway by chance alone. Raw values were determined based on the number of hits and the total number of compounds in the pathway. The pathway impact was found using relative betweenness centrality pathway topology analysis, which calculates metabolite importance based on their position in the pathways.