Inhibition of DNA Repair Protein Ku70 in High-Glucose Environment Aggravates the Neurotoxicity Induced by Bupivacaine in SH-SY5Y Cells

Figure 3

Long-term high-glucose conditions inhibited the Ku70 expression and induced more severe apoptosis. (a) Ku70 mRNA levels in SH-SY5Y cells’ serum starved for 24 h followed by incubation with increasing glucose concentrations (25, 50, or 100 mM) for 2 days. (b) Ku70 mRNA levels in SH-SY5Y cells’ serum starved for 24 h followed by incubation with increasing glucose concentrations (25, 50, or 100 mM) for 7 days. (c) Western blotting bands and data of the Ku70 expression in SH-SY5Y cells’ serum starved for 24 h and were then incubated with 50 mM glucose for 2, 4, or 7 days. (d) Western blotting bands and data of the cleaved caspase-3 expression in SH-SY5Y cells’ serum starved for 24 h and were then incubated with 50 mM glucose for 2, 4, or 7 days. Values are the mean ± SEM of n = 3. compared with the untreated control. compared with the group incubated with 50 mM glucose for 4 days.

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