Inhibition of DNA Repair Protein Ku70 in High-Glucose Environment Aggravates the Neurotoxicity Induced by Bupivacaine in SH-SY5Y Cells

Figure 4

High glucose inhibited the Ku70 expression and enhanced bupivacaine-induced apoptosis. (a) Western blotting bands and data of the Ku70 expression in SH-SY5Y cells. (b) Western blotting bands and data of the cleaved caspase-3 expression in SH-SY5Y cells. Con: untreated SH-SY5Y cells; HG: SH-SY5Y cells exposed to 50 mM glucose for 7 days; Bup: SH-SY5Y cells treated with 1.0 mM bupivacaine for 24 h; HG + Bup: SH-SY5Y cells incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h; LV5 + HG + Bup: SH-SY5Y cells transfected vector (LV5) incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h; LV5-Ku70 + HG + Bup: SH-SY5Y cells transfected overexpression lentivirus (LV5-Ku70) incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h. Values are the mean ± SEM of n = 3. compared with Con. compared with HG and Bup. compared with HG + Bup and LV5 + HG + Bup.

(a)

(b)