Inhibition of DNA Repair Protein Ku70 in High-Glucose Environment Aggravates the Neurotoxicity Induced by Bupivacaine in SH-SY5Y Cells

Figure 5

High glucose enhanced bupivacaine-induced ROS production. The ROS production as detected by redox-sensitive fluorescent dye DCFH-DA. Summarized data show the intracellular ROS content, expressed as the ratio of mean fluorescence intensity of treated groups to that of untreated controls. (a) Con: untreated SH-SY5Y cells. (b) HG: SH-SY5Y cells exposed to 50 mM glucose for 7 days. (c) Bup: SH-SY5Y cells treated with 1.0 mM bupivacaine for 24 h. (d) HG + Bup: SH-SY5Y cells incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h. (e) LV5 + HG + Bup: SH-SY5Y cells transfected vector (LV5) incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h. (f) LV5-Ku70 + HG + Bup: SH-SY5Y cells transfected overexpression lentivirus (LV5-Ku70) incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h. (g) Values are the mean ± SEM of n = 6. compared with Con. compared with HG and Bup.

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