Next-Generation Sequencing Panel Analysis of Clinically Relevant Mutations in Circulating Cell-Free DNA from Patients with Gestational Trophoblastic Neoplasia: A Pilot Study

Table 2

Details of overlapping mutations.

No.	Gene	Transcript	Patients	Exons	Nucleotide variation	Type
1	BMPR1A	NM_004329	P1	Exon10	c.1001 T > C	SNV
			P2	Exon10	c.1001 T > C	SNV
			P8	Exon10	c.1001 T > C	SNV
2	LRP1B	NM_018557	P1	Exon39	c.6221 A > C	SNV
			P9	Exon43	c.7034 T > G	SNV
			P10	Exon39	c.6221 A > C	SNV
3	ERCC4	NM_005236	P4	Exon9	c.1853 T > A	SNV
			P10	Exon2	c.304 A > T	SNV
4	FGF14	NM_001321937	P1	Exon4	c.424-3delT	Splicing
			P3	Exon4	c.424-3delT	Splicing
5	HSP90AA1	NM_005348	P2	Exon5	c.836_838delAGA	Deletion
			P3	Exon5	c.836_838delAGA	Deletion
6	KAT6A	NM_006766	P1	Exon2	c.282 A > T	SNV
		NM_006766	P2	Exon16	c.3218_3219insGGA	Insertion
7	KMT2D	NM_003482	P2	Exon13	c.4059_4061delGGA	Deletion
		NM_003482	P5	Exon10	c.1301 T > G	SNV
8	MAP3K1	NM_005921	P1	Exon14	c.2917_2918insCTC	Insertion
			P3	Exon20	c.4487delG	Deletion
9	RANBP2	NM_006267	P5	Exon16	c.2275 G > A	SNV
			P8	Exon20	c.6680 A > G	SNV
10	ZNF217	NM_006526	P1	Exon3	c.1645 G > A	SNV
			P10	Exon3	c.1645 G > A	SNV