Involvement of the G-Protein-Coupled Receptor 4 in the Increased Expression of RANK/RANKL/OPG System and Neurotrophins by Nucleus Pulposus Cells under the Degenerated Intervertebral Disc-Like Acidic Microenvironment

Table 1

Primers used for RT-PCR.

Gene	Primer sequences
GPR4	F: 5-CGGGTCAAGACAGCAGTAG-3
	R: 5-ACAAGGTGTGGTTAGCGAT-3
RANK	F: 5-ACGTGGACCCTTGCCCCAGT-3
	R: 5-ACTGGCCACCAGGGGAGCTT-3
RANKL	F: 5-GACAGGCACGGACTCGTA-3
	R: 5-CGCTCATGCTAGTCGTCTA-3
OPG	F: 5-TACAGCATCACTACGTAGGAC-3
	R: 5-ACGTCATGCGATCACATATCG-3
NGF	F: 5-GCCCACTGGACTAAACTTCAGC-3
	R: 5-CCGTGGCTGTGGTCTTATCTC-3
BDGF	F: 5-GGTCACAGTCCTGGAGAAAG-3
	R: 5-GTCTATCCTTATGAACCGCC-3
NT-3	F: 5-GATCCAGGCGGATATCTTGA-3
	R: 5-AGCGTCTCTGTTGCCGTAGT3
MMP-3	F: 5-CCTCTGATGGCCCAGAATTGA-3
	R: 5-GAAATTGGCCACTCCCTGGGT-3
MMP-13	F: 5-TGACTATGCGTGGCTGGAA-3
	R: 5-AAGCTGAAATCTTGCCTTGGA-3
ADAMTS-5	F: 5-AGTTTGCCTACCGCCATTG-3
	R: 5-CATTCTGTCCCATCTGTAACCTT-3
18S-RNA	F: 5-GAATTCCCAGTAAGTGCGGGTCATA-3
	R: 5-CGAGGGCCTCACTAAACCATC-3