Involvement of the G-Protein-Coupled Receptor 4 in the Increased Expression of RANK/RANKL/OPG System and Neurotrophins by Nucleus Pulposus Cells under the Degenerated Intervertebral Disc-Like Acidic Microenvironment

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td class="align_left">Target</td><td class="align_center">Sequences (5<span class="nowrap"><svg height="11.4859pt" id="M45" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>-</span>3<span class="nowrap"><svg height="11.4859pt" id="M46" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>)</span></td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td class="align_left">shRNA1</td><td class="align_center">AGGAGAAAGTCAAGATCAAAC</td></tr><tr><td class="align_left">shRNA2</td><td class="align_center">TCTTTCATGACGAGCTCTTTC</td></tr><tr><td class="align_left">shRNA3</td><td class="align_center">TAGCCTTCACCAGCCTCAATT</td></tr><tr><td class="align_left">Scramble sequence</td><td class="align_center">CCTAAGGTTAAGTCGCCCTCG</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>DNA sequences GPR4-shRNA and scramble sequence.</div>

BioMed Research International

tab2

Table 2

Table 2: Involvement of the G-Protein-Coupled Receptor 4 in the Increased Expression of RANK/RANKL/OPG System and Neurotrophins by Nucleus Pulposus Cells under the Degenerated Intervertebral Disc-Like Acidic Microenvironment 

Table 2 | Involvement of the G-Protein-Coupled Receptor 4 in the Increased Expression of RANK/RANKL/OPG System and Neurotrophins by Nucleus Pulposus Cells under the Degenerated Intervertebral Disc-Like Acidic Microenvironment 