Development of Human Vectored Brucellosis Vaccine Formulation: Assessment of Safety and Protectiveness of Influenza Viral Vectors Expressing Brucella Immunodominant Proteins in Mice and Guinea Pigs

Table 2

Level of protective efficacy of vaccines assessed by the isolation rate of Brucella from the spleens of mice challenged with the virulent strain B. melitensis 16M.

Groups	Vaccine samples	No. of animals	Brucella titer, log10 CFU/g spleen ()	Protection unit (log10)	Significance to control group
					(+) control	(-) control
1	80-Omp16	5		2.79	>0.05	<0.05
2	80-L7/L12	5		2.17	>0.05	>0.05
3	80-Omp19	5		2.2	>0.05	>0.05
4	80-SOD	5		2.24	>0.05	>0.05
5	124-Omp16	5		2.46	>0.05	>0.05
6	124-L7/L12	5		2.40	>0.05	>0.05
7	124-Omp19	5		1.78	>0.05	>0.05
8	124-SOD	5		2.60	>0.05	<0.05
9	80-Omp16+L7/L12	5		2.1	>0.05	>0.05
10	80-Omp19+SOD	5		1.7	>0.05	>0.05
11	80-Omp16+Omp19	5		2.04	>0.05	>0.05
12	80-L7/L12+SOD	5		2.30	>0.05	>0.05
13	80-Omp16+L7/L12+Omp19+SOD	5		2.75	>0.05	<0.05
14	124-Omp16+L7/L12	5		2.38	>0.05	>0.05
15	124-Omp19+SOD	5		2.23	>0.05	>0.05
16	124-Omp16+Omp19	5		2.69	>0.05	<0.05
17	124-L7/L12+SOD	5		1.60	>0.05	>0.05
18	124-Omp16+L7/L12+Omp19+SOD	5		2.70	>0.05	<0.05
19	B. melitensis Rev.1	5		2.68	—	<0.05
20	Control (PBS)	5		—	<0.05	—

Table legend: log₁₀ protection units were obtained by subtracting the mean log₁₀ CFU of the control (PBS) group from the mean of log₁₀ CFU for the experimental group and for the positive control group. (+) control: animals vaccinated with B. melitensis Rev.1 commercial vaccine. (-) control: animals inoculated with PBS. vs. PBS control group, B. melitensis Rev.1 vs. vaccine groups. Statistical analysis was performed using a one-way ANOVA (Tukey’s multiple comparison test).