miR-1258 Regulates Cell Proliferation and Cell Cycle to Inhibit the Progression of Breast Cancer by Targeting E2F1

Figure 6

miR-1258 regulates the proliferation, apoptosis, and cell cycle of BC cells by targeting E2F1. BC cells were cotransfected with NC-mimic+oe-NC, miR-1258-mimic+oe-NC, and miR-1258-mimic+oe-E2F1. (a) The mRNA expression of E2F1 was tested by qRT-PCR. (b) The protein expression of E2F1 was tested by WB. (c–f) Effects of miR-1258/E2F1 on BC cell (c) proliferation, (d) growth, (e) apoptosis, and (f) cell cycle were detected by MTT, colony formation, and flow cytometry. The results were representative of at least 3 independent experiments, .

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