Neuroepithelial Cell Transforming Gene 1 Acts as an Oncogene and Is Mediated by miR-22 in Human Non-Small-Cell Lung Cancer

Figure 4

miR-22 weakens the proliferation and migration of NSCLC cells and enhances cell apoptosis. (a) Quantitative reverse transcription polymerase chain reactions (qRT-PCRs) showed that miR-22 mimics and inhibitors successfully take effect in both A549 and H1299 cells. (b) CCK-8 assay indicated that miR-22 mimics decreased cell’s vitality, while miR-22 inhibitors increased cell’s vitality (n = 3). (c) Similarly, EdU staining confirmed that miRNA-22 mimics resulted in a reduced cell’s proliferation, while miR-22 inhibitors increased that effect (n = 4). (d) miR-22 mimics attenuated NSCLC cell migration in A549 and H1299 cells (n = 3). (e) Western blot was used to analyze EMT, and β-actin was considered as a loading control (n = 3). (f) Induction of cell apoptosis confirmed by flow cytometry (n = 4). (g) Apoptosis associated protein was analyzed using western blot, and β-actin was used as a control (n = 3). .

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