Research Article
Aberrant ALOX5 Activation Correlates with HER2 Status and Mediates Breast Cancer Biological Activities through Multiple Mechanisms
Figure 2
The HER2 overexpression promotes the ALOX5 expression and activity in breast cancer cells. (a) Representative Western blotting image of HER2 and ALOX5 in MCF-7, SKBR3, and BT-474 cells. (b) SKBR3 and BT-474 have higher 5-HETE level than MCF-7. (c) Representative Western blotting image of HER2, ALOX5, and ALOX5AP in SKBR3 and BT-474 cells after HER2 siRNA knockdown. Cells were harvested for analysis at 48 h posttransfection. (d) HER2 knockdown significantly decreases the 5-HETE level in breast cancer cells. (e) Representative Western blotting image of p-HER2, HER2, ALOX5, and ALOX5AP in SKBR3 and BT-474 cells exposed to 10 μM zileuton. (f) Herceptin (20 μg/ml) significantly decreases the 5-HETE level in breast cancer cells. , compared to MCF-7, siControl, or control.
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