Aberrant ALOX5 Activation Correlates with HER2 Status and Mediates Breast Cancer Biological Activities through Multiple Mechanisms

Figure 4

ALOX5 inhibition suppresses RhoA, focal adhesion, and PI3K/Akt/mTOR in breast cancer cells. ALOX5 inhibition via siRNA (a) and zileuton (b) significantly decreases the RhoA activity in SKBR3 cells. Representative image (c) and quantification (d) of Western blotting of p-paxillin, p-FAK, E-cadherin, vimentin, p-PI3K, p-Akt, and p-mTOR in SKBR3 cells exposed to zileuton or transfected with siALOX5. Western blot analysis was performed using SKBR3 cells after 1-day zileuton treatment or the transfected cells at 48 h posttransfection. , compared to control or siControl.

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