Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis

Figure 3

DCs are activated through AhR ligands. DCs were stimulated by with 10 nM TCDD (TCDD group) and 10 μM CH223191+10 nM TCDD (CH+TCDD group) for 48 h. DCs of HC and PBC patients were obtained and analyzed the expression of AhR and CYP1A1 by qRT-PCR (a) and Western Blots (b). The expression of AhR and CYP1A1 were significantly increased after TCDD treatment. After TCDD stimulated DCs, the expressions of IL-22, IDO1, CCL-4, IL-12, and IL-23 of the TCDD group were higher than other groups by qRT-PCR (c) and ELISA (d). Results are presented as . Significantly higher than the control, (, , and ).

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