Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
TCDD-treated DCs altered the expression of IFN-γ, IL-17, T-bet, and RORγt in naïve CD4+ T cells. DCs were cocultured with PBC patients or HC naïve CD4+ T cells for 3 days. (a) Naïve CD4+ T cells surface markers were detected by FACS and the proportion of CD4+ CD45RA+ naïve CD4+ T cells reached 97.8%. (b) CCK-8 detected the naïve CD4+ T cell proliferation differences between different groups. (c)After coculture, the expressions of IFN-γ, IL-17, T-bet, and RORγt of the PBC DC+PBC T cell group were higher than those of the HC DC+HC T cell group in RNA level. (d) Then, AhR-activated DCs altered the expression of T-bet and RORγt in naïve CD4+ T cells at protein levels using ELSA kit. Data are expressed as . Significantly higher than control, (, , and ).