Research Article
Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain
Table 1
PBD with enzyme activity response as affected by the four independent variables.
| | Run | X1
Glucose (g/L) | X2
Yeast extract (g/L) | X3
Malt extract (g/L) | X4
CaCO3 (g/L) | Response CO activity (U/mL) |
| | 1 | 6.0 (+1) | 2.0 (−1) | 12.0 (+1) | 0.5 (−1) | 21.732 | | 2 | 6.0 (+1) | 6.0 (+1) | 8.00 (−1) | 4.0 (+1) | 17.713 | | 3 | 2.0 (-1) | 6.0 (+1) | 12.0 (+1) | 0.5 (−1) | 10.081 | | 4 | 6.0 (+1) | 2.0 (−1) | 12.0 (+1) | 4.0 (+1) | 21.940 | | 5 | 6.0 (+1) | 6.0 (+1) | 8.00 (−1) | 4.0 (+1) | 17.713 | | 6 | 6.0 (+1) | 6.0 (+1) | 12.0 (+1) | 0.5 (−1) | 18.000 | | 7 | 2.0 (-1) | 6.0 (+1) | 12.0 (+1) | 4.0 (+1) | 11.597 | | 8 | 2.0 (-1) | 2.0 (−1) | 12.0 (+1) | 4.0 (+1) | 16.761 | | 9 | 2.0 (-1) | 2.0 (−1) | 8.00 (−1) | 4.0 (+1) | 15.245 | | 10 | 6.0 (+1) | 2.0 (−1) | 8.00 (−1) | 0.5 (−1) | 21.460 | | 11 | 2.0 (-1) | 6.0 (+1) | 8.00 (−1) | 0.5 (−1) | 8.6070 | | 12 | 2.0 (-1) | 2.0 (−1) | 8.00 (−1) | 0.5 (−1) | 12.870 |
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