Research Article
Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain
Table 2
Analysis of variance of CO activity versus glucose, yeast extract, malt extract, and CaCO3.
| Source | DF | Adj. SS | Adj. MS | F-value | value |
| Model | 4 | 223.723 | 55.931 | 101.06 | 0.000 | Linear | 4 | 223.723 | 55.931 | 101.06 | 0.000 | Glucose (X1) | 1 | 156.942 | 156.942 | 283.56 | 0.000 | Yeast extract (X2) | 1 | 57.628 | 57.628 | 104.12 | 0.000 | Malt extract (X3) | 1 | 3.524 | 3.524 | 6.37 | 0.040 | CaCO3 (X4) | 1 | 5.629 | 5.629 | 10.17 | 0.015 | Residual error | 7 | 3.874 | 0.553 | | | Lack-of-fit | 6 | 3.874 | 0.646 | | | Pure error | 1 | 0.000 | 0.000 | | | Corrected total | 11 | 227.597 | | | | Model summary | S | R-seq. | R-seq. (Adjusted) | R-seq. (Predicted) | | | 0.743952 | 98.30% | 97.33% | 95.00% | |
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