Research Article
Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain
Table 5
Analysis of variance of BBD for CO production model using S. rochei NAM-19.
| | Source | DF | Adj. SS | Adj. MS | F-value | value |
| | Model | 14 | 1586.49 | 113.21 | 7500.08 | 0.000 | | Linear | 4 | 8.72 | 2.181 | 144.34 | 0.000 | | X1 | 1 | 6.36 | 6.360 | 420.90 | 0.000 | | X2 | 1 | 0.88 | 0.878 | 58.19 | 0.000 | | X3 | 1 | 4.31 | 4.313 | 285.44 | 0.000 | | X4 | 1 | 0.00 | 0.000 | 0.03 | 0.870 | | Square | 4 | 110.93 | 27.733 | 1835.51 | 0.000 | | X12 | 1 | 16.93 | 16.933 | 1120.70 | 0.000 | | X22 | 1 | 57.20 | 57.200 | 3785.77 | 0.000 | | X32 | 1 | 1.62 | 1.617 | 107.04 | 0.000 | | X42 | 1 | 7.46 | 7.457 | 493.51 | 0.000 | | 2-Way interaction | 6 | 61.34 | 10.224 | 676.67 | 0.000 | | X1 X2 | 1 | 2.82 | 2.822 | 186.80 | 0.000 | | X1 X3 | 1 | 27.41 | 27.405 | 1813.80 | 0.000 | | X1 X4 | 1 | 9.00 | 9.000 | 595.66 | 0.000 | | X2 X3 | 1 | 0.02 | 0.022 | 1.49 | 0.246 | | X2 X4 | 1 | 21.73 | 21.734 | 1438.47 | 0.000 | | X3 X4 | 1 | 0.36 | 0.360 | 23.83 | 0.000 | | Residual error | 12 | 0.18 | 0.015 | | | | Pure error | 2 | 0.00 | 0.000 | | | | Corrected total | 26 | 1586.68 | | | | | Model summary | S | R-seq. | R-seq. (Adjusted) | R-seq. (predicted) | | | 0.122920 | 99.99% | 99.98% | 99.93% |
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