Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated Streptomyces rochei NAM-19 Strain

Table 6

Regression analysis of BBD with model coefficients for total CO production by S. rochei NAM-19.

Term	Effect	Coefficient	SE coefficient	T-value	value
Constant		−16.44	2.63	−6.26	0.000
X1	10.423	5.212	0.254	20.52	0.000
X2	3.390	1.695	0.222	7.62	0.000
X3	11.485	5.742	0.340	16.89	0.000
X4	−0.078	−0.039	0.235	−0.17	0.870
X1^2	0.8909	0.4455	0.0133	33.48	0.000
X2^2	−1.6375	−0.8187	0.0133	−61.53	0.000
X3^2	−0.2753	−0.1377	0.0133	−10.35	0.000
X4^2	−0.5912	−0.2956	0.0133	−22.22	0.000
X1X2	0.4200	0.2100	0.0514	13.67	0.000
X1X3	−1.3087	−0.6544	0.0514	−42.59	0.000
X1X4	0.7500	0.3750	0.0514	24.41	0.000
X2X3	0.0375	0.0187	0.0514	1.22	0.246
X2X4	1.1655	0.5828	0.0514	37.93	0.000
X3X4	−0.1500	−0.0750	0.0514	−4.88	0.000