Asiatic Acid Interferes with Invasion and Proliferation of Breast Cancer Cells by Inhibiting WAVE3 Activation through PI3K/AKT Signaling Pathway

Table 2

RNA primer volume.

Components	Volume	Final concentration
Total RNA	2 μg	2 μg/rxn
Oligo dT primer (10 μM)	1 μl	0.5 μM
dNTPs (10 mM each)	1 μl	500 μM
RNase-free water	Up to 14.5 μl