Research Article

Asiatic Acid Interferes with Invasion and Proliferation of Breast Cancer Cells by Inhibiting WAVE3 Activation through PI3K/AKT Signaling Pathway

Table 4

PCR reaction solution.

Reagent componentsVolumeFinal concentration

2X qPCR MasterMix10 μl1X
Forward primer (10 μM)0.6 μl300 nM
Reverse primer (10 μM)0.6 μl300 nM
cDNA (2 μg)0.4 μl
Nuclease-Free WaterUp to 20 μl