Asiatic Acid Interferes with Invasion and Proliferation of Breast Cancer Cells by Inhibiting WAVE3 Activation through PI3K/AKT Signaling Pathway

Table 5

PCR amplification conditions.

Steps	Number of cycles	Temperature	Time	Detection
Predenaturation	1	95°C	10 min	Off
Denaturation	40	95°C	5 sec	Off
Annealing and extension		60°C	30 min	On