Research Article
RCAN1 Inhibits BACE2 Turnover by Attenuating Proteasome-Mediated BACE2 Degradation
Figure 2
RCAN1 inhibits BACE2 turnover. (a) HEK293 cells and (b) HRNLM cells were transfected with pBACE2-mycHis. 48 hours after transfection, cells were treated with 100 μg/mL CHX for 0, 2, 4, 8, or 12 h. Cell lysates were resolved by 10% SDS-PAGE. 9E10 antibody was used to detect myc-tagged BACE2 protein. β-Actin served as an internal control. (c) Quantification of BACE2 levels. Values represent .
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