OSCC Exosomes Regulate miR-210-3p Targeting EFNA3 to Promote Oral Cancer Angiogenesis through the PI3K/AKT Pathway

Figure 2

CAL27 CM promotes angiogenesis of HUVEC by elevating miR-210 expression. (a–c) Migration and tube formation assays showed that CAL27 CM could promote migration and tube formation ability of HUVECs. , . (d) The CCK-8 assay showed that CAL27 CM could promote HUVEC proliferation. . (e) RT-PCR showed that the expression of miR-210 in HUVECs was elevated after being treated with CAL27 CM. . (f) By being treated with miR-210 mimics, inhibitor, and their negative control (NC) groups, miR-210 was changed accordingly. , . (g–j) The CCK-8 assay, migration assay, and tube formation assay showed that compared to NC groups, miR-210 mimics could improve HUVEC angiogenesis while the inhibitor could restrain this capacity. Scale bar, 50 μm. , .

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