Acacetin Suppresses IL-1β-Induced Expression of Matrix Metalloproteinases in Chondrocytes and Protects against Osteoarthritis in a Mouse Model by Inhibiting NF-κB Signaling Pathways

Figure 3

Acacetin inhibits the expression of MMP-1, MMP-3, and MMP-13 in IL-1β-stimulated human and murine primary chondrocytes. (a) The mRNA expression levels of MMP-1, MMP-3, and MMP-13 in chondrocytes treated with IL-1β (10 ng/mL) and acacetin (H-acacetin, 6.25 μM; L-acacetin, 3.125 μM) for 24 h. (b) Western blot showing MMP-1, MMP-3, and MMP-13 activity in chondrocytes treated with IL-1β (10 ng/mL) and H- or L-acacetin for 48 h. (c) Relative intensity of protein bands normalized to β-actin (). , , and .

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