Nrp-1 Mediated Plasmatic Ago2 Binding miR-21a-3p Internalization: A Novel Mechanism for miR-21a-3p Accumulation in Renal Tubular Epithelial Cells during Sepsis

Figure 2

Ago2 and miR-21a-3p levels in TECs stimulated with differently treated plasma. (a) Quantitative analysis of the results from ELIA for Ago2 and RT-PCR for miR-21a-3p of plasma with or without treatments. (b) Quantitative analysis of RT-PCR results of miR-21a-3p of TECs treated with different plasma. (c) Representative results of flow cytometry for Ago2 in TECs treated with different plasma. (d) Quantitative analysis of the results of flow cytometry for Ago2 in TECs treated with different plasma. (e) Representative Western Blot results of Ago2 in TECs treated with different plasma ( vs. the control; Ctrl: control; NP: normal plasma; 12 h: septic plasma from rats 12 hours after CLP; 12 h+Ab: septic plasma treated with Ago2 antibody; 12 h+IP: septic plasma treated with Ago2 immunoprecipitation).

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