Nrp-1 Mediated Plasmatic Ago2 Binding miR-21a-3p Internalization: A Novel Mechanism for miR-21a-3p Accumulation in Renal Tubular Epithelial Cells during Sepsis

Figure 5

Exogenous Ago2 in TECs with different treatments. (a) Representative fluorescent results of cytoplasm Ago2 of TECs with different treatments. (b) Representative flow cytometry results of Ago2 in TECs with different treatments. (c) Quantitative analysis of flow cytometry results of Ago2 in TECs with different treatments. (d) Representative Western Blot results of cytoplasm Ago2 in TECs with different treatments. (e) Representative Western Blot results of cytoplasm His residue at 99 Kd in TECs with different treatments. (f) Representative results of immunoprecipitation of Ago2 binding His residue of TECs in the control and exogenous Ago2-treated groups. (g) Quantitative analysis of RT-PCR results of intracellular miR-21a-3p of TECs with different treatments ( vs. the control; Ctrl: control; SiNrp-1: Nrp-1 siRNA transfection).

(a)

(b)

(c)

(d)

(e)

(f)

(g)