An Inhibitor of DRP1 (Mdivi-1) Alleviates LPS-Induced Septic AKI by Inhibiting NLRP3 Inflammasome Activation

Figure 3

Lipopolysaccharide (LPS) treatment can induce the elevation of DRP1 expression in mouse renal tubular epithelial (TCMK-1) cells. (a) Cell viability was tested in the presence of concentrations of LPS (0, 0.1, 1, 5, and 10 μg/ml) in 12 hours of culture. (b) Cell viability was tested in relation to different culture time points (0, 1, 6, and 12 hours). (c) Western blot assay of DRPI expression in mitochondria after different concentrations of administration of LPS (0, 0.1, 1, 5, and 10 μg/ml) in 12 hours of culture. (d) Western blot assay of DRPI expression in mitochondria in different culture time points (0, 1, 6, and 12 hours) of 5 μg/ml LPS administration. (e) GTPase activity assay of DRP1. for each group in all experiments. The data are presented as . versus the concentration of LPS is 0 μg/ml or versus time point is 0 hours. ^# versus control group.

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