An Inhibitor of DRP1 (Mdivi-1) Alleviates LPS-Induced Septic AKI by Inhibiting NLRP3 Inflammasome Activation

Figure 5

Knocking down the NLRP3 gene by siRNA/siNLRP3 transfection can attenuate the expression of pyroptosis pathway proteins and inhibit apoptosis. (a) Western blot protein expression bands of the NLRP3 inflammasome-related proteins, NLRP3, GSDMD, cl.Caspase-1, IL-1β, and IL-18 ( for each group). (b) Semiquantitative analysis of Western blot protein expression bands of the NLRP3 inflammasome-related proteins ( for each group). (c) Flow cytometry analysis of cell apoptosis. Q2 represents the number of cell necrosis and Q4 represents the number of apoptotic cells. The total number of cells is 10000. (d) Quantitative analysis of flow cytometry evaluation of cell apoptosis. Annexin V-positive and 7-AAD-negative (Annexin V⁺7-AAD^-) cells were defined as apoptotic cells. The data are presented as . versus control-treated cells. ^# versus LPS-treated cell non-siNLRP3-transfected group.

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