Huaier Granule Combined with Tegafur Gimeracil Oteracil Potassium Promotes Stage IIb Gastric Cancer Prognosis and Induces Gastric Cancer Cell Apoptosis by Regulating Livin
Huaier polysaccharides promote gastric cancer cell apoptosis with dose and duration dependence. (a) Cellular morphological evaluation of SGC-7901 and BGC-823 cells after treatment with 0, 50, and 100 μg/mL of Huaier polysaccharide for 72 h, observed by using an inverted phase contrast microscope at a 100x magnification. (b) CCK assays showed the OD value ( nm) after treatment with 0, 50, and 100 μg/mL Huaier polysaccharide for 24, 48, and 72 h, respectively. (c) Colony formation assay analysis of SGC-7901 and BGC-823 cell proliferation after treatment with 0, 50, and 100 g/mL Huaier polysaccharide, respectively. (d) Annexin-FITC/PI assay and flow cytometry to analyze the SGC-7901 and BGC-823 cell apoptosis after treatment with 0, 50, and 100 μg/mL Huaier polysaccharide for 24, 48, and 72 h, respectively.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.