Huaier Granule Combined with Tegafur Gimeracil Oteracil Potassium Promotes Stage IIb Gastric Cancer Prognosis and Induces Gastric Cancer Cell Apoptosis by Regulating Livin

Figure 2

Huaier polysaccharides promote gastric cancer cell apoptosis with dose and duration dependence. (a) Cellular morphological evaluation of SGC-7901 and BGC-823 cells after treatment with 0, 50, and 100 μg/mL of Huaier polysaccharide for 72 h, observed by using an inverted phase contrast microscope at a 100x magnification. (b) CCK assays showed the OD value ( nm) after treatment with 0, 50, and 100 μg/mL Huaier polysaccharide for 24, 48, and 72 h, respectively. (c) Colony formation assay analysis of SGC-7901 and BGC-823 cell proliferation after treatment with 0, 50, and 100 g/mL Huaier polysaccharide, respectively. (d) Annexin-FITC/PI assay and flow cytometry to analyze the SGC-7901 and BGC-823 cell apoptosis after treatment with 0, 50, and 100 μg/mL Huaier polysaccharide for 24, 48, and 72 h, respectively.

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