Huaier Granule Combined with Tegafur Gimeracil Oteracil Potassium Promotes Stage IIb Gastric Cancer Prognosis and Induces Gastric Cancer Cell Apoptosis by Regulating Livin

Figure 4

Huaier polysaccharides promote SGC-7901 cell apoptosis by regulating the expression of Livin. (a) Relative expression of Livin expression in SGC-7901 treated with 100 μg/mL Huaier polysaccharide and processed after 72 h at the mRNA level. (b, c) Relative expression of Livin and caspase 3 expression in SGC-7901 treated with 100 μg/mL Huaier polysaccharide and processed after 72 h at the protein level. (d) Relative expression of Livin in SGC-7901 cells transfected with the Livin mimic and treated with 100 μg/mL Huaier polysaccharide, after 72 h at the mRNA level. (e, f) Relative expression of Livin expression in SGC-7901 cells transfected with the Livin mimic and treated with 100 μg/mL Huaier polysaccharide, after 72 h at the protein level. (g) CCK8 assay analysis of the OD value ( nm) in each group. (h) Annexin V-FITC/PI assay and flow cytometry to analyze the SGC-7901 cell apoptosis in each group and processing after 72 h.

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