Silencing of ELK3 Induces S-M Phase Arrest and Apoptosis and Upregulates SERPINE1 Expression Reducing Migration in Prostate Cancer Cells

Figure 1

Silencing ELK3 inhibited the proliferation, adhesion, colony-forming, and migration of DU145 cells in vitro. (a) DU145 cells were transfected with siRNAs for 72 h, the expression of ELK3 and ACTIN were detected by western blotting (up panel); the effect of ELK3 knockdown on DU145 cell proliferation was examined by CCK-8 assay (lower panel). (b) Effect of ELK3 knockdown on cell adhesion. (c) Effect of ELK3 knockdown on cell colony-forming. (d) Transwell assay was performed to test the effect of ELK3 knockdown on cell migration ability. Cells were fixed with 4% paraformaldehyde and stained with crystal violet, then dissolved in 33% v/v acetic acid and measured the absorbance at 570 nm (c, d). Data were averaged from three parallel experiments and are given as mean ± SD. , , vs NC-siRNA.

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