Induction of SOCS Expression by EV71 Infection Promotes EV71 Replication

Figure 3

Early induction of SOCS gene transcription is an IFN-independent manner. (a–c) RD cells were infected with EV71 () or stimulated with human IFNβ for the indicated time points. The mRNA levels of SOCS1 and SOCS3 (a, c), IFNα and IFNβ (b), and ISG56 (c) were analyzed by RT-PCR. (d) Knockdown of IFNAR1 in HEK293T cells was performed with shRNA and determined by RT-PCR. (e–h) The effect of EV71 on SOCS1/3 expression in IFNAR1 knockdown cells. The mRNA levels of SOCS1 and SOCS3 (e, f) and IFNα and IFNβ (g, h) were analyzed by RT-PCR. All the data were representative of at least three independent repeats. The statistical significance analyses were performed using two-tailed unpaired -test (NS: no significance; , , and ).

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