Induction of SOCS Expression by EV71 Infection Promotes EV71 Replication

Figure 4

EV71 infection activates SOCS expression through the NF-κB pathway and inhibits IFN-induced STAT3 phosphorylation. (a) RD cells were stimulated with or without IFNβ for 6 h and then infected with or without EV71 virus for 24 h. The expression of SOCS3, as well as STAT3 phosphorylation, was analyzed by western blot. The relative expressions of SOCS3, p-STAT3, and STAT3 were normalized to those of lane 1. (b) RD cells were treated with NF-κB inhibitor BAY11-7082 for 1 h and then infected with EV71 virus at the indicated time points. SOCS3 expression was detected by RT-PCR. The data are representative of at least three independent repeats. The statistical significance analyses were performed using two-tailed unpaired -test (NS: no significance; , , and ). (c) Schematic diagram of the EV71 virus activating SOCS gene expression and inhibiting the JAK/STAT signaling pathway.

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