Cell-to-Cell Spread of Dengue Viral RNA in Mosquito Cells

Figure 2

Identification of DENV RNAs and C189 in stratified fractions of cell lysate by sucrose centrifugation. (a) Viral proteins and RNA were detected from fractions collected by sucrose centrifugation from DENV2-infected C6/36 cells transfected with the C189-overexpressing vector and infected by DENV2. A total of 23 fractions were collected in top-to-bottom order, from which viral E protein was identified from fractions 7~17 while C protein appeared in fractions 5~17 and C189 was detected mainly from fractions 5~12. This implied that progeny virions actually exist and colocalize with C189. (b) Viral RNAs, including positive- (+) or negative- (-) sense, were detected from the selected fractions (7~18 for the positive sense and 8~16 for the negative sense). In particular, (-) RNA was identified in fractions 8~10 in parallel with the distributions of viral E and C proteins and C189. This shows that DENV2 virions, proteins, and RNAs are contained in virus-induced C189-VCs. M: DNA markers.

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