Cell-to-Cell Spread of Dengue Viral RNA in Mosquito Cells

Figure 3

Detection of viral RNA and E proteins from immunoisolated C189-VCs collected from DENV2-infected C6/36 cells. (a) C189-VCs were collected from C6/36 cells (DENV2-infected and C189-transfected) via immunoisolation. Subsequently, both (+) and (-) viral RNAs were detected in the flow sample; i.e., unprecipitated cell lysates. Both strands of viral RNA were identified from immunoisolated cell components (IP), suggesting that C189-VCs actually contain viral RNA, both (+) and (-), during DENV2 infection in C6/36 cells. (b) The Western blot result showed that viral E protein can be detected from IP (arrow), further indicating that virions and/or viral components are contained within C189-VCs.

(a)

(b)