Research Article
Kaempferol Protects Cell Damage in In Vitro Ischemia Reperfusion Model in Rat Neuronal PC12 Cells
Figure 2
KFL ameliorated OGD-induced mitochondrial apoptosis in PC12 cells. (a) Apoptosis was measured using a TUNEL assay kit. (b) Protein expression of cleaved caspase 3, caspase 9, and caspase 7 was determined using western blot. (c and d) Caspase 3 and caspase 9 activities were measured using commercial kits. (e and f) mRNA expression of Bax and Bcl-2 was measured using real-time PCR. .
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |