USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1/AKT Pathway

<div>USP46 inhibited cell proliferation during DNA damage in lung cancer cells. (a, b) Proliferation of H1299 (a) and A549 (b) cells overexpressing USP46 (oeUSP46) and control vectors (oeNC) exposed to radiation was measured by CCK-8. <svg height="9.65783pt" id="M35" style="vertical-align:-0.3499298pt" version="1.1" viewbox="-0.0498162 -9.3079 139.8 9.65783" width="139.8pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M861 0V28C774 35 771 41 768 147L759 509C756 612 762 614 851 622V650H681L449 149L221 650H57V622C148 613 153 609 144 479L130 271C123 166 117 123 111 88C104 46 85 34 26 28V0H259V28C192 35 169 42 167 90C166 130 166 173 170 256L185 541H187L411 7H431L675 555H679L683 147C683 41 680 35 598 28V0H861Z"></path></g><g transform="matrix(.013,0,0,-0.013,11.244,0)"><path d="M380 106C343 72 306 56 265 56C195 56 116 112 115 248C235 252 361 262 377 265C396 269 400 277 400 297C400 374 333 449 250 449H249C198 449 144 421 103 376S37 269 37 201C37 88 109 -12 232 -12C263 -12 332 6 395 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(c, d) <i>γ</i>-H2AX foci formation in H1299 (c) and A549 (d) oeNC and oeUSP46 cells after exposure to radiation was assessed by IF at the indicated time points after 4 Gy radiation exposure. (e) <i>γ</i>-H2AX foci formation in USP46 knockdown H446 cells after exposure to 4 Gy radiation was assessed by IF.</div>

BioMed Research International

fig4

Figure 4

Figure 4: USP46 Inhibits Cell Proliferation in Lung Cancer through PHLPP1/AKT Pathway 