Research Article
Skin Pigmentation Differences between Mongolian, Korean, and Uzbekistan Ancient Human DNA Samples
Table 1
Primers and HybProbes used for the amplification and detection of rs1545397.
| | Primers/Probes | Sequence (5-3) | Tm (°C)a | Note |
| | Primers | | | | | rs1545397_F | TGGAATTGGATACTGACAATGGTTG | 64.4 | Bouakaze et al. 2009 | | rs1545397_R | CATGGGGGAGAGAGAATGACTCAG | 67.7 | Bouakaze et al. 2009 | | Probes | | | | | rs1545397_A | ATTCACACAACATAAAATTTATCTTGCAA-FLb | 60 | This study complementary sequence | | rs1545397_S | LC Red640c-ATTATATCATTCAGTGTATTTTAGTATATT-PHd | 55(T)
50.5(A) | Channel 640 |
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a: calculated by using LC PDS software (version 2.0). b: FL, fluorescein. c: LC Red640, LightCycler dye Red640. d: PH, phosphate.
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