Identification of the Functional Domain of HPIV3 Matrix Protein Interacting with Nucleocapsid Protein

Figure 1

Interaction of N and M. (a, b) After 293T cells were transfected with the corresponding plasmids, the protein was immunoprecipitated with anti-HA (a) or anti-Myc (b). The protein level was detected by WB. (c) N-Myc and HA-M plasmids were used to transfect 293T cells alone or together, and the protein expression in cell lysate and VLP was detected by WB. (d) After cotransfecting HeLa cells with plasmids encoding N-Flag and HA-M, N was stained with anti-Flag and AF488-conjugated fluorescent secondary antibody, and M was stained with anti-HA and AF568-conjugated fluorescent secondary antibody. Immunofluorescence was observed under a microscope.  μm.

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