Identification of the Functional Domain of HPIV3 Matrix Protein Interacting with Nucleocapsid Protein

Figure 2

The C-terminal truncation mutant of M has a defect in its ability to encapsulate N into VLP. (a) N-Myc, HA-M, HA-MΔC120, and HA-MΔC170 plasmids were used to transfect 293T cells alone or together, and the proteins in cell lysate and VLP were detected by WB. (b) Relative germination index. The data is averaged from the results of three independent experiments. . (c) N-Myc, HA-M, HA-MΔC190, and HA-MΔC210 plasmids were individually or cotransfected into 293T cells, and the proteins in cell lysate and VLP were detected by WB. (d) Relative germination index. The data is the average of the results of three independent experiments. .

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