Identification of the Functional Domain of HPIV3 Matrix Protein Interacting with Nucleocapsid Protein

Figure 4

MC190 effectively encapsulates N into its VLP by interacting with N. (a) N-Myc plasmid was cotransfected into 293T cells with HA-M or HA-MC190 plasmids, respectively. After transfection, cells and VLPs were collected and protein expression was detected by WB. (b) The relative ability of M and MC190 to encapsulate N into VLP. The data are from the average of three independent experiments, ns: . (c) After transfection with N-Myc and HA-M or HA-MC190 plasmids, 293T cells were collected and immunoprecipitated with anti-Myc, and protein expression was detected by WB. Asterisks indicate specific proteins detected. (d) The relative ability of the M+N group or the MC190+N group to interact with N. The data are from the average of three independent experiments, ns: not significant (). (e) After transfection with N-Flag plasmid and HA-M or HA-MC190 plasmid, the HeLa cells were subjected to immunofluorescence analysis.  μm.

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