BioMed Research International / 2020 / Article / Fig 3

Research Article

1H-Indole-3-Carbonyl-Thiazole-4-Carboxylic Acid Methyl Ester Blocked Human Glioma Cell Invasion via Aryl Hydrocarbon Receptor’s Regulation of Cytoskeletal Contraction

Figure 3

Effects of ITE on the spreading, protrusion attachment, and migration of glioma cells in 2D and 3D collagen matrix. (a–f) U87MG cells were plated in the type I collagen matrix, settled overnight, treated with either ITE or DMSO, and recorded by time-lapse photography: (a, b) Cells were treated with DMSO or ITE for 20 hr. (c, d) Cells were treated with DMSO or ITE for 1 hr. (e–f) Cells were treated with DMSO or ITE for 2 hr. (g–j) U87MG cells in 2D culture were treated with DMSO or ITE for 18 hr and recorded for 2 hr. (g, h) Overlap of photos taken at the beginning (cell edge colored in red) and end of the 2 hr (cell edge colored in green) using ImageJ. (i, j) Videos of U87MG cells in 2D culture.
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