[Retracted] 1H-Indole-3-Carbonyl-Thiazole-4-Carboxylic Acid Methyl Ester Blocked Human Glioma Cell Invasion via Aryl Hydrocarbon Receptor’s Regulation of Cytoskeletal Contraction

Figure 4

MYH9 expression level and cell migration ability. (a) Cells were treated with ITE for 18 hr, and mRNA levels of MYH9 were determined by RT-PCR using GAPDH as a reference gene. (b) MYH9 protein levels were assessed in U87MG by western blots. The bar graph summarizes the semiquantitation of the western blot data. (c–e) Overexpression of MYH9-mCherry fusion protein by transfection of the U87MG cells. (c) MYH9 gene overexpressed in U87MG cell after 36 h transfection; the left panel is the bright field, the middle panel is red fluorescence from mCherry, and the right panel is the merged picture. (d, e) MYH9 mRNA and protein level were determined by RT-PCR and western blot using GAPDH as a reference gene () in MYH9 transfected an empty vector (PCMV) or untransfected cells. (f) ITE reduced MYH9 mRNA level in U87MG cells with normal or elevated MYH9 level. MYH9 or empty vector-transfected cells were treated with either ITE or DMSO for 18 hr, and MYH9 mRNA levels were determined. (g) ITE’s effect on the migration ability of the U87MG cells that overexpressing MYH9. Either MYH9 plasmid or empty vector-transfected cells were treated with various doses of ITE or DMSO for 20 hr. The whole experiments were replicated 3 times, and the area of migration was measured using 2 replicate wells in each batch. : various ITE concentrations compared with DMSO treatment in vector; ^#: various ITE concentration compared with DMSO in MYH9 overexpression ( and ). (g) Correlation between MYH9 mRNA level and cell migration area in the U87MG cells.

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