Exosomal miRNA-215-5p Derived from Adipose-Derived Stem Cells Attenuates Epithelial–Mesenchymal Transition of Podocytes by Inhibiting ZEB2

Figure 4

Effects of miR-215-5p loaded in ADSCs-Exos on HG-mediated migration of MPC5 cells. (a) The expression of miR-215-5p was measured by qRT-PCR in ADSC treated with miR-215-5p inhibitor. (b) The level of miR-215-5p was analyzed by qRT-PCR in exosomes from ADSCs treated with miR-215-5p inhibitor. (c) Cell migration was evaluated by Transwell assay in HG-induced MPC5 cells treated with exosomes with low miR-215-5p expression and miR-215-5p mimic. (d) The number of migratory cells was analyzed by ImageJ software, and the expression of miR-215-5p was measured by qRT-PCR. (e) Tracking of the PKH26-labeled ADSC-Exos with low miR-215-5p expression or normal miR-215-5p expression in HG-induced MPC5 cells. ; .

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