|
Method | Materials | Muscle | Results | Ref |
|
Detergent | Sodium dodecyl sulfate Triton X-100 Sodium deoxycholate | Porcine skeletal | Suitable decellularization using only SDS Production of a too thin gel for hydrogel | [53] |
Porcine major psoas | Suitable decellularization | [54] |
Rat gastrocnemius | Unaltered ECM anisotropy and chemical components | [55] |
Rat hind limb | Suitable decellularization Loss of specific sarcolemma proteins | [56] |
Bovine tail | Suitable decellularization | [57] |
Human flexor digitorum superficialis | ECM decellularized with unaltered composition | [51] |
|
Detergent/enzymatic | Det + deoxyribonuclease Det + ribonuclease A Det + Trypsin | Rat, rabbit, human skeletal | Triton+trypsin resulted more effective in removing cellular material and maintaining the 3D fiber networks | [50] |
Rat diaphragm | Suitable decellularization ECM conservation, tissue micro- and ultra-architecture preservation | [58] |
Porcine skeletal | Suitable decellularization and gelation using trypsin/EDTA, Triton X-100, and Triton X-100/SDS | [53] |
Human rectus femoris and supraspinatus | Suitable decellularization | [59] |
Porcine rectus abdominal | ECM decellularized Altered ECM proteins levels | [60] |
Rat diaphragm | Suitable decellularization | [61, 62] |
Rabbit hind leg | ECM decellularized with unaltered collagen, proteins, and sGAG levels Loss of stiffness | [63] |
Rabbit lower limb | Suitable decellularization Altered collagen levels Increased pore sizes | [64] |
Porcine skeletal | Suitable decellularization Altered collagen, GAGs, and elastin levels No proper structural stability | [65] |
Porcine lower limb | ECM decellularized with altered collagen, elastin, and sGAG levels | [66] |
Human flexor digitorum superficialis | ECM not completely decellularized | [51] |
|
Detergent/alcohol | Det + isopropanol Det + methanol | Porcine psoas | Suitable decellularization | [67, 68] |
Porcine longissimus dorsi | Suitable decellularization Difference in fat and protein compositions depending on harvesting conditions | [69] |
|
Detergent/enzymatic/Alcohol | | Porcine rectus abdominis | ECM decellularized Altered ECM proteins levels | [70] |
Porcine tibialis anterior | Suitable decellularization | [71, 72] |
|
No detergent/no enzymatic | Latrunculin B Potassium chloride Potassium iodide | Rat hind limb | Suitable decellularization Loss of VEGF | [56] |
Human flexor digitorum superficialis | ECM not completely decellularized | [51] |
|