Investigating Tunneling Nanotubes in Cancer Cells: Guidelines for Structural and Functional Studies through Cell Imaging

Figure 3

Formation of TNT through directed filopodia-like protrusion in HBEC-3 cells. HBEC-3 cells were cultured on a glass surface, and time-lapse was acquired through phase contrast microscopy (dry 20x, DMI 6000 video microscopy, Leica Microsystems). Red arrows indicate sequential steps of TNT formation. Scale bar, 20 μm.