Pomegranate Seeds Extract Possesses a Protective Effect against Tramadol-Induced Testicular Toxicity in Experimental Rats

Figure 5

DNA ploidy image cytometry of Tr-treated testicular tissues and cotreated with PgSE. Adult and adolescent rat testes were examined via Feulgen staining. The nuclear integrated optical density (OD) which is the cytometric equivalent of DNA content was measured. The DNA histograms were classified based on the amount of DNA as haploid (<1.5c), diploid (1.5–2.5c), triploid (2.5–3.5c), and tetraploid (3.5–4.5c). The Tr-treated group had diminished the triploid cell population in both ages. Scale bars: 100 μM.