Astragalus Polysaccharide Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Oxidative Damage and Mitochondrial Dysfunction

Figure 4

APS attenuates cisplatin-induced oxidative stress. (a) Immunohistochemical staining of 3-NT expression in mice kidney tissues (original magnification ×200; bar = 250 μm). (b) T-SOD activity and (c) GSH-Px activity in mice kidney tissues. Data are means ± SEM (n = 5). versus the control group; , versus the cisplatin alone-treated group. (d) APS reduced the intracellular ROS generation in HK-2 cells induced by cisplatin treatment. Separated and merged histograms of the DCFH-DA-stained HK-2 cells by flow cytometry. (e) APS significantly reduced the mitochondrial superoxide accumulation in HK-2 cells induced by cisplatin treatment. MitoSOX™ Red staining of HK-2 cells with different treatments (original magnification ×200; bar = 250 μm). (f) Quantification of MitoSOX Red-positive cells in 6 random fields. Data are means ± SEM (n = 6). versus the control group; versus the cisplatin alone-treated group.

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